High Transfection Efficiencies with Minimal or No Toxicity

QuickStep Transfection Kit

Cat: No.: EM-101-006, Unit Size: Trial Size 0.2mL
Cat: No.: EM-101-007, Unit Size: 1mL

• Quick 1-step, 1-hour procedure
• High efficiency transfection
• Optimized for medium with serum
• Low toxicity
• Broad cell range for commonly used cell lines such as HeLa, COS, 293, and NIH3T3
• Transfection for siRNA and plasmids

Use QuickStep Transfection Kit for high efficiency transfection for siRNA and plasmids. The QuickStep transfection reagent is a liposomal formulation of a polycationic lipid specifically designed for a 1-step, 1 hour transfection method. Use the same medium for both transfection and cell culture maintenance because QuickStep transfection reagent is optimized for the presence of serum which makes it less toxic to cells. No more serum-free media just for transfections. Say good-bye to multi-step, multi-day transfection procedures and hello to our 1-step, 1-hour QuickStep Transfection Kit.

SURE FECTOR

Cat.: No. EM-101-001, Unit Size: 1mL
Cat. No.: EM-101-001s, Unit Size: Trial Size Set (0.2mL each)

SureFECTOR is a liposomal formulation of a polycationic lipid and a neutral, non-transfecting lipid compound. SureFECTOR has been used successfully to transfect DNA into HepG2, COS, PC12, NIH/3T3, Jurkat, primary rat and hamster hepatocytes, primary rat cardiomyocytes, primary male rat germ cells, primary chick retinal neurons, primary rat fibroblasts, primary quail myoblasts, primary human keratinocytes, and tracheobronchial cells. Peak activities were achieved using 1-4 mg SureFECTOR per mg DNA on the tested cell lines grown in 35 mm dishes. Therefore, 1 milliliter of SureFECTOR is sufficient for 250-1000 transfection. No significant toxicity was observed at the concentrations used for optimal activity on the cell lines tested. SureFECTOR is provided as a sterile suspension in water at a concentration of 1 mg/ml.

Protocol
MSDS
Certificates of Analysis
Ordering information

UniFECTOR

Cat. No.: EM-101-002, Unit Size: 1mL
Cat. No.: EM-101-003s, Unit Size: Trial Size Set (0.2mL each)

UniFECTOR is a liposomal formulation of a polycationic lipid and a neutral, non-transfecting lipid compound. UniFECTOR has been used successfully to transfect DNA or RNA into CHO-K1, COS, NIH/3T3, LNCaP, T-24, HEK/293, C2C12, Schneider (Drosophila), SF-9, human bone marrow endothelial cells (HBMEC), primary rat aortic smooth muscle, primary rabbit myoblasts, and primary human keratinocytes. Peak activities were achieved using 2-6 mg UniFECTOR per mg DNA on the tested cell lines grown in 35 mm dishes. Therefore, 1 milliliter of UniFECTOR is sufficient for 160-500 transfections. No significant toxicity was observed at the concentrations used for optimal activity on the cell lines tested. UniFECTOR is provided as a sterile suspension in water at a concentration of 1 mg/ml.

Protocol
MSDS
Certificates of Analysis
Ordering information

SiFECTOR

Cat. No.: EM-101-004, Unit Size: Trial Size 0.2mL
Cat. No.: EM-101-005, Unit Size: 1mL

siFECTOR is a liposomal formulation of a polycationic lipid and a neutral, non-transfecting lipid compound. SiFECTOR has been used successfully to transfect siRNA into CHO-K1, COS, NIH3T3, LNCaP, T-24, 293, C2C12, Schneider (Drosophila), SF-9, human bone marrow endothelial cells (HBMEC), primary rat aortic smooth muscle, primary rabbit myoblasts, and primary human keratinocytes. Peak activities were achieved using 2-6 mg siFECTOR per mg siRNA on the tested cell lines grown in 35 mm dishes. Therefore, 1 mL of siFECTOR is sufficient for 160-500 transfections. No significant toxicity was observed at the concentrations used for optimal activity on the cell lines tested. siFECTOR is provided as a sterile suspension in water at a concentration of 1 mg/ml.

Protocol
MSDS
Certificates of Analysis
Ordering information