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Quantify Expression with Real Time Amplification |
B-Bridge provides several convenient real-time pre-mixes as well as offering several optimized application kits from Biotools based on their propriety LionProbes™ method.
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Biotools Optimized Real Time PCR Kits
Biotools offers several optimized kits for detection and quantification microorganisms using Real-Time PCR. All of these kits utilize Biotools proprietary LionProbe™ technology and can be provided in a format that incorporates their unique Gelification process where the components are stabilized at room temperature.
Currently, Biotools offers the following Kits:
2X Real Time PCR Master Mixes
Convenient, effective, and quantitative amplification and detection of a variety of DNA targets such as genomic DNA, cDNA and plasmid DNA.
Available with four different MgCl2 concentrations.
Contain the Low ROX reference dye as a standard.
Include B-Bridge’s Hot Start DNA polymerase that decreases background from non-specific amplification.
Real Time Master Mix variations contain suitable concentrations of the SYBR Green intercalating dye.
Real Time Master Mix variations are available with dUTP substituted for dTTP and added uracil-N-glycosylase (UNG). |
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Basic Real Time Master Mix
For use with various fluorescent probes or intercalating dyes
Low ROX Reference dye
MgCl2 (3 mM, 6 mM, 7 mM, 10 mM)dNTP’s
Hot Start DNA Polymerase
Optimized buffer
Green Real Time Master Mix
Premixed with SYBR Green Dye for convenient SYBR-based real-time PCR
Low ROX Reference dye
SYBR® Green DNA dye
MgCl2 (3 mM, 6 mM, 7 mM, 10 mM)
dNTP’s
Hot Start DNA Polymerase
Optimized buffer
dUTP Real Time PCR Master Mix
Prevents carry over contamination with uracil-N-glycosylase (UNG) and dUTP
Low ROX Reference dye
MgCl2 (3 mM, 6 mM, 7 mM, 10 mM)
dATP, dCTP, dGTP
dUTP
Uracil-N-glycosylase
Hot Start DNA Polymerase
Optimized buffer
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