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ELISAs

Adipokine ELISAs
Adiponectin
- Adiponectin
- Protocol
- Specifications
- FAQ
- Kit References
Leptin
Leptin Receptor
Resistin

Incubation Center




Specifications


Human Adiponectin ELISA Kit Cat. # K1001-1

Components:
25X Wash Solution 1 Bottle (40 mL)
Sample Pre-treatment Solution 1 Bottle (10 mL)
5X Sample Diluent 1 Bottle (50 mL)
Primary Antibody-Coated Plate (Mouse anti-human monoclonal) 1 Plate (96-well)
Adiponectin Standard (recombinant) 1 Vial (2 mL; 12 ng/mL)
Secondary Antibody Solution (Rabbit anti-human polyclonal) 1 Bottle (12 mL)
Detection Antibody Concentrate (HRP-conjugated goat anti-rabbit 1 Vial (0.1 mL)
Detection Antibody Diluent 1 Bottle (15 mL)
Substrate A 1 Bottle (7.5 mL)
Substrate B 1 Bottle (7.5 mL)
Stop Solution 1 Bottle (15 mL)
Plate Sealers 6 sealers
Storage:
Store all components at 2-8oC. Do not freeze.
Sample Types:
Serum/Plasma, Adipocyte Cell Extracts, Adipocyte Conditioned Media
Minimum Detectable Limit:
23.4pg/ml of adiponectin
Range:
0.375ng/ml to 12.0ng/ml
Incubation Time:
3 hrs & 15 min



Mouse Adiponectin ELISA Kit Cat. # K1002-1

Components:
25X Wash Solution 1 Bottle (40 mL)
5X Sample Diluent 1 Bottle (50 mL)
Primary Antibody-coated Plate (Rabbit anti-mouse polyclonal) 1 Plate (96-well)
Adiponectin Standard (recombinant) 1 Vial (2 mL; 8 ng/mL)
Biotinyated Secondary Antibody Solution (Rabbit anti-mouse polyclonal) 1 Bottle (12 mL)
HRP-Conjugated Streptavidin 1 Vial (0.1 mL)
Streptavidin Diluent 1 Bottle (15 mL)
Substrate A 1 Bottle (7.5 mL)
Substrate B 1 Bottle (7.5 mL)
Stop Solution 1 Bottle (15 mL)
Plate Sealers 6 sealers
Storage:
Store all components at 2-8oC. Do not freeze.
Sample Types:
Serum/Plasma, Adipocyte Cell Extracts, Adipocyte Conditioned Media
Minimum Detectable Limit:
15.6 pg/ml of adiponectin
Range:
0.25 ng/ml to 8.0 ng/ml
Incubation Time:
3 hrs & 15 min



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