B-Bridge - Adipokine ELISAs, Resistin - the Product of the RSTN Gene

ELISAs

Adipokine ELISAs

Adiponectin

Leptin

Leptin Receptor

Resistin

- Resistin
- Specifications

Human
- User Manual (pdf)
- MSDS (pdf)

Mouse
- User Manual (pdf)
- MSDS (pdf)

Resistin - the Product of the RSTN Gene

Resistin, a product of the resistin (RSTN) gene, is a peptide hormone belonging to the class of cysteine-rich secreted proteins (monomeric peptides that contain 11 cysteine residues) referred to as the RELM family, and is also described as adipose tissue-specific secretory factor (ADSF) or found in inflammatory zone 3 (FIZZ3). Mouse resistin is expressed as a 114 amino acid signal peptide is cleaved before its secretion. Mouse resistin circulates in blood as a homodimeric protein consisting of two 94 amino acid polypeptides, which are disulfide-linked via Cys26.

Resistin may be an important link between obesity and insulin resistance. Mouse resistin, specially produced and secreted by adipocyte, acts on skeletal muscle myocytes, hepatocytes and adipocytes themselves so that it reduce their sensitivity to insulin. Steppan et al. have suggested that resistin suppressed the ability of insulin to stimulate glucose uptake. They have also suggested that resistin was present at elevated levels in blood of obese mice, and was down regulated by fasting and by anti-diabetic drugs. Wey et al., on the other hand, suppressed in obesity and is stimulated by several ati-diabetic drugs.

Other studies have shown that mouse resistin increases during the differentiation of adipocytes, but it also seems to inhibit adipogenesis. In contrast, the human adipogenic differentiation is likely to be associated with a down regulation of resistin gene expression.

References for Resistin:

Steppan C.M., Lazar M.A.: Resistin and obesity-associated insulin resistance. TRENDS in Endocrinology and Metabolism. 13 (1), 18-23, (2002)

Banerjee R.R., Lazar M.A.: Dimerization of resistin and resistin - like moleules is determined by a single cystein. The Journal of Biological Chemistry. 276, 25970-25973, (2001)

Kim KH. et al.: A Cysteine-rich Adipose Tissue-specific Secretory Factors Inhibits Adipocte Differentiation. The Journal of Biological Chemistry. 276 (14), 11252-11256, (2001)

Steppan C.M. et al.: The Hormone Links Obesity to Diabetes. Nature. 409, 307-312, (2001)

Way J.M. et al.: Adipose Tissue Resistin Expression Is Severly Suppressed in Obesity and Stimulated by Peroxisome Proliferator-activated Receptor Agonists. The Journal of Biological Chemistry. 276 (28), 25651-25653, (2001)

Hartman H.B., Lazar M.A. at al.: Mechanisms Regulating Adipocyte Expression of Resistin. The Journal of Biological Chemistry. 277 (22), 19754-19761, (2002)

Fasshauer M. at al.: Tumor Necrosis Factor Is a Negative Regulator of Resistin Gene Expression and Secretion in 3T3-L1 Adipocytes. Biochemical and Biophysical Research Communications. 208, 1027-1031, (2001)

Steppan C.M. at al.: A Family of Tissue-specific Resistin-like Molecules. Proc. Natl. Acad. Sci. USA. 98 (2), 502-506, (2001)

Juan CC. at al.: Suppressed Gene Expression of Adipocyte Resistin in an Insulin-resistant Rat Model Probably by Elevated Free Fatty Acids. Biochemical and Biophysical Research Communications. 289, 1328-1333, (2001)