| Protocol Overview
Human Kit PDF | Mouse/Rat Kit PDF
The B-Bridge Human and Mouse/Rat Adiponectin ELISA Kits measure the concentration of adiponectin from serum, adipocytes, or conditioned medium. After adding samples and a serially diluted standard to an appropriate number of wells in the microtiter plate, you follow the procedure outlined in the diagram below to perform the assay.
1st Reaction: Adiponectin in the sample is bound by the primary adiponectin antibody immobilized in the well.
2nd Reaction: After washing, the secondary antibody is added to each well and allowed to incubate. The secondary antibody binds to the adiponectin trapped in the well during the 1st Reaction.
3rd Reaction: After washing, a conjugate of horseradish peroxidase (HRP) is added to each well and allowed to incubate. The HRP-conjugated reagent recognizes and binds to the secondary antibody trapped in the well in the 2nd Reaction.
Color Development: After washing, the colorimetric substrate for the enzyme is added to all wells and incubated. The color development is terminated by the addition of a stop solution. The intensity of the color is measured at 450 nm. The concentrations of the samples tested are calculated using the absorbance values of the adiponectin standard solutions assayed at the same time.
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